Device

Part:BBa_K101008:Design

Designed by: Sarah Hendrickson   Group: iGEM08_Minnesota   (2008-07-28)


Biological Comparator Inducible by IPTG and aTc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2466
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2293


Design Notes

One of the main considerations dealt with was the size of this part and whether it would be possible to successfully ligate a part of this size into an E.coli base vector. Most other considerations were dealt with during the design of the four smaller composite parts that this device is composed of.


Source

This part is composed from four composite parts: K101003, K101004, K101005, and K101006.

References